Consensus sequence for Escherichia coli heat shock gene promoters.

Consensus sequence for Escherichia coli heat shock gene promoters.

We have identified promoters for the Escherichia coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5. Transcription from each promoter is heat-inducible in vivo, and each is recognized in vitro by RNA polymerase containing sigma 32, the sigma factor encoded by rpoH (htpR) but not by RNA polymerase containing sigma 70. We compared the sequences of the heat shock ...

Selection of Escherichia coli heat shock promoters towards their 1

Regulation of the promoters and transcripts of rpoH, the Escherichia coli heat shock regulatory gene.

In Escherichia coli the product of the rpoH (htpR) gene, sigma 32, directs RNA polymerase to initiate transcription from heat shock promoters at all temperatures. Transcription of the heat shock genes is increased when cells are exposed to high temperatures because of increased transcription initiation by sigma 32-RNA polymerase. As a step toward understanding the regulation of the heat shock r...

Escherichia coli heat-shock proteins

lstituto di Microbiologia’ and lstituto di Clinica Dermosif ilopaticaz, Seconda Universita degli Studi di Napoli, Facolta di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2,801 38 Napoli, Italy Bacterial heat-shock proteins (HSPs) from Escherichia co/i (GroES, GroEL and DnaK) were studied for their ability to induce by themselves the expression and release of tumour necrosis factor-a ...

Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.

We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma 32-dependent promoter and is thus part of the heat...